Chromatography

Kromatográfia

A tantárgyleírás hatályossága

Hatályosság kezdete:

2026. March 21.

Hatályosság vége:

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Subject name (Hungarian, English)

Kromatográfia

Chromatography

Subject code

BMEVESAAL11

Subject type

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Training Level

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Course types and hours (weekly/semester)

Course type	lecture	tutorial	laboratory
hours (weekly)	2	0	0
type (linked/independent)	—	—	—

Assessment type

vizsga

Credits

Subject coordinator

Dr. Tóth Blanka

position: egyetemi docens

contact: toth.blanka@vbk.bme.hu

Responsible department

Szervetlen és Analitikai Kémia Tanszék

https://www.ch.bme.hu/

Faculty

Vegyészmérnöki és Biomérnöki Kar

Subject website

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Primary curriculum type

—

Direct prerequisites – Strong prerequisite

BMEVESAAL17 (Analitikai kémia labor)

Direct prerequisites – Weak prerequisite

none

Direct prerequisites – Parallel prerequisite

none

Direct prerequisites – Milestone prerequisite

none

Direct prerequisites – Exclusion

none

Objectives

Programme

Role of chromatography in analytical chemistry. Classification of chromatographic systems based on the quality stationary and mobile phase.

Basic definitions used in chromatography and their relations in the separation.

Role of retention, kinetic efficiency and selectivity in the separation.

Prerequisites for gas chromatographic analysis. Relation of vapor pressure and retention in gas chromatography. Efficiency differences of packed and capillary columns and their applications. Basics of gas-solid and gas-liquid chromatography and their applications.

Mobile phases in gas chromatography. Effect of mobile phase quality on the separation. Van Deemter and Golay equations and thier explanations.

Stationary phases and their characterisation. Choosing the appropriate stationary phase based on analyte structure and boiling point.

Instrument design. Packed column and capillary column gas chromatographs. Instrument parts and their function.

GC detectors: operation, classification, analytical characterisation.

Qualitative and quantitative analysis in gas chromatography.

Basic theory of liquid chromatography. Parameters influencing resolution. Application of Van Deemter and Knox equations in LC. Classification of fluid chromatographic techniques based on the forces resulting solute migration. Basics of kinetic theory. Recent developments in liquid chromatography.

Characterization of packed columns. Effect of particle diameter and its distribution on resolution. Typical mobile phases and their effect on resolution.

Normal phase liquid chromatography (NP-HPLC). Packings: silicagel, aluminum-oxide, modified silicagels. Mobile phases. Parameters influencing retention, selectivity and resolution.

Reversed phase liquid chromatography (RP-HPLC). Packings: silicagel based, carbon based and other types. Effect of mobile phase composition, pH, ionic strenght and temparture on the separation.

Application of secondary equilibria for increased selectivity. Ion-supression and ion-pair chromatography in RP-HPLC.

Basics of ion-exchange chromatography (IE-HPLC). Organic and inorganic packings an their applications.

Methodologies for the separation large molecules. Size exclusion chromatography: gel chromatography, gel permeation.

Basics of gradient elution.

Instrumentation in HPLC: pumps, detectors, system control

Important applications and some relating sample preparation methods (LLE, LSE, SFE)

- pharmaceutical industry

- enviromental protection

- biotechnology

- biochemistry

- food industry

- other areas

Recent developments in liquid chromatography. Capillary columns and monolith packings. Hyphenated techniques (e.g. LC-MS)

The subject lays emphasis on the basics and applications of chromatographic analysis: theoretical background and practice will be discussed in order to develop skills for method development and application of hyphenated techniques.

Learning outcomes

Ez a tantárgy a KKK rendeletben meghatározott, következő kompetenciák fejlesztését szolgálja:

Knowledge

No learning outcomes recorded.

Skills

No learning outcomes recorded.

Attitudes

No learning outcomes recorded.

Autonomy and responsibility

No learning outcomes recorded.

Oktatási módszertan

Substantive literature search and laboratory practice, consultation with the supervisor.

Tanulástámogató anyagok

Online források

Environmental Analysis, R. N. Reeve. John Wiley and Sons Ltd. 1994.;

Recommended preliminary knowledge for completing the subject

Knowledge type competencies

(azon előzetes ismeretek összessége, amelyek megléte nem kötelező, de a tantárgy eredményes teljesítését nagyban elősegíti)

nincs

Skill type competencies

(azon előzetes képességek és készségek összessége, amelyek megléte nem kötelező, de a tantárgy eredményes teljesítését nagyban elősegíti)

nincs

Recommended (non-compulsory) preliminary competencies

(azon ajánlott (nem kötelező) előzetesen megszerzendő kompetenciák összessége, amelyek jelentősen hozzájárulnak a tantárgy eredményes teljesítéséhez)

Basics of classical and instrumental analysis methods and environmental analysis

General rules

Requirements: Fullfillment of the following tasks: literature search, design and cost estimation of the analysis, sampling, sample preparation, measurement, evaluation and reporting. Presenting a talk on the project and writing a test. Re-takes: The test can be repeated. Consultations: By previously fixed appiontment with the lecturer.

Assessment methods

In-term assessments

No detailed assessments provided.

Weight of in-term assessments

No weights provided.

Exam-period assessments

No detailed assessments provided.

Weight of exam elements

No weights provided.

Grade calculation

No grade thresholds provided.

Attendance requirements

No attendance requirements provided.

Rules for retake and resubmission

Not provided.

Short description

Not provided.

Detailed description

Name: Post: Department: Dr. Jenő Fekete professor emeritus Inorg. and Anal. Chem.

Recommended courses

30 hours for laboratory work and 40 hours for literaure search, reporting, consultation, etc.

Workload to complete the subject

No workload breakdown provided.

Validity of subject requirements

Requirements valid from:

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Requirements valid until:

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Curriculum placement

Faculty	Program	Curriculum	Curriculum type	Primary
Default Faculty	vegyészmérnöki	Vegyészmérnöki alapképzési szak tanterve	kötelező	nem
Default Faculty	Default Program	Default Curriculum	—	nem