Chromatography

Kromatográfia

A tantárgyleírás hatályossága

Hatályosság kezdete:

2026. March 21.

Hatályosság vége:

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Subject name (Hungarian, English)

Kromatográfia

Chromatography

Subject code

BMEVESAM503

Subject type

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Training Level

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Course types and hours (weekly/semester)

Course type	lecture	tutorial	laboratory
hours (weekly)	2	0	0
type (linked/independent)	—	—	—

Assessment type

vizsga

Credits

Subject coordinator

Dr. Tóth Blanka

position: egyetemi docens

contact: toth.blanka@vbk.bme.hu

Responsible department

Szervetlen és Analitikai Kémia Tanszék

https://www.ch.bme.hu/

Faculty

Vegyészmérnöki és Biomérnöki Kar

Subject website

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Primary curriculum type

—

Direct prerequisites – Strong prerequisite

none

Direct prerequisites – Weak prerequisite

none

Direct prerequisites – Parallel prerequisite

none

Direct prerequisites – Milestone prerequisite

none

Direct prerequisites – Exclusion

none

Objectives

Programme

Introduction. Short history of chromatography. Grouping the basic chromatographic methods. Theoretical possibilities of chromatographic separation (frontal, displacement, elution separation). Chromatographic bases: stationary phase, mobile phase (eluent), retention, resolution, selectivity, efficiency, capacity, partition coefficient.

The basic questions of chromatography: flow in particulate filling and empty tube. Chromatographic bases. Expectations for chromatographic analysis. The eluent flow. Theoretical description of gas chromatographic separation: equilibrium theory, plate theory, velocity theory. Characterization of the concentration distribution (elution curve).

Dependence of retention on flow rate and temperature. Thermodynamic approximation of retention. The Van Deemter equation and the Golay equation, its role in optimizing the separation. Characterization of separation (resolution, separation number, peak capacity, required plate number, analysis time

Types of gas chromatography columns (adsorption and distribution columns, thin and thick film columns, high internal diameter, standard and small inner diameter columns). Gas chromatographic stationary phases. adsorbents

Gas chromatographic stationary phases. Gas chromatographic retention index and GC polarity. The aspects of choosing stationary phases. Tune the selectivity. Column Connections. Programmed temperature GC. Two-dimensional GC

Composition of gas chromatography systems. Gas system (eluents), sample injector systems. Gas chromatographic detectors (TCD, FID, AFID, Ar and He detectors, ECD, DID, PID, VUV, MS)

Quantitative analysis methods (calibration, addition, internal standard methods). Analytical applications

Categorization of liquid chromatographic methods. Specify materials that can be measured by different methods. Normal Phase Chromatography. Stationary phases, mobile phases, requirements.

Reversed Phase Liquid Chromatography. Stationary phases, mobile phases, requirements. The role of pH control in RP-HPLC

General Description of Ion Chromatography Techniques. Ion exchange, ion pair, ion-exclusion chromatography. Use of suppressor columns, special detectors

Methods for separating hydrophilic materials: HILIC, polar endcapped stationary phases. Description of gradient elution method

Composition of HPLC systems. High pressure pumps, sample injectors, detectors (UV, FL, RI, ELSD, CAD, MS).

Possibilities of increasing chromatographic efficiency. Fast LC. Chiral separations, stationary phases. 2D HPLC

Comparison of different chromatographic columns, aspects of column selection. Mapping the causes of bad peak shape. Practical applications

Education of chemical engineers possessing op-to-date knowledge in chromatography enabling them to solve analytical tasks and to adapt new techniques.

Learning outcomes

Ez a tantárgy a KKK rendeletben meghatározott, következő kompetenciák fejlesztését szolgálja:

Knowledge

The students are familiar with gas and liquid chromatographic analytical solutions that are used in the chemical industry and are generally used for chemical processes. They are familiar with the construction and operating conditions of devices, chromatographic systems used in these methods. They are familiar with the methods of data collection and literature search needed to develop chromatographic methods.

Skills

They can adapt new measurement methods, can carry out the validation of these with guidance by peers, can improve methods. They can carry out chromatographic laboratory measurements, operate the devices, and evaluate the measurement results.

Attitudes

They strive to get to know the latest chromatographic methods. They can use this knowledge in practice to apply the most effective methods with the lowest possible use of resources (chemicals, time, working hours).

Autonomy and responsibility

Performs independently chromatographic measurements in compliance with the quality and safety requirements. Monitors the operation of chromatographic instruments and recognizes common mistakes.

Oktatási módszertan

lecture

Tanulástámogató anyagok

Online források

Introduction to Modern Liquid Chromatography, 3rd Edition by L.R. Snyder, J.J. Kirkland and J.W. Dolan

Recommended preliminary knowledge for completing the subject

Knowledge type competencies

(azon előzetes ismeretek összessége, amelyek megléte nem kötelező, de a tantárgy eredményes teljesítését nagyban elősegíti)

nincs

Skill type competencies

(azon előzetes képességek és készségek összessége, amelyek megléte nem kötelező, de a tantárgy eredményes teljesítését nagyban elősegíti)

nincs

Recommended (non-compulsory) preliminary competencies

(azon ajánlott (nem kötelező) előzetesen megszerzendő kompetenciák összessége, amelyek jelentősen hozzájárulnak a tantárgy eredményes teljesítéséhez)

Basics of classical and instrumental analysis methods and environmental analysis

General rules

Requirements: Attendance on 75% of the lectures Exam Re-takes: The exam can be repeated. Consultations: By previously fixed appiontment with the lecturer.

Assessment methods

In-term assessments

No detailed assessments provided.

Weight of in-term assessments

No weights provided.

Exam-period assessments

No detailed assessments provided.

Weight of exam elements

No weights provided.

Grade calculation

No grade thresholds provided.

Attendance requirements

No attendance requirements provided.

Rules for retake and resubmission

Not provided.

Short description

Not provided.

Detailed description

Name: Post: Department: Dr. József Balla associate professor Inorg. and Anal. Chem. Dr. Blanka Tóth assistant professor Inorg. and Anal. Chem.

Recommended courses

28 hours lectures 50 hours learning at home

Workload to complete the subject

No workload breakdown provided.

Validity of subject requirements

Requirements valid from:

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Requirements valid until:

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Curriculum placement

Faculty	Program	Curriculum	Curriculum type	Primary
Vegyészmérnöki és Biomérnöki Kar	vegyészmérnöki	Vegyészmérnöki mesterképzési szak tanterve	kötelezően választható	nem
Default Faculty	Default Program	Default Curriculum	—	nem